Fatma Abd EL-Lateef Gharib
Department of Botany, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt
ABSTRACT
When leaf and petiole explants of P. nervosum were cultured on Murashige and Skoog medium supplemented with various combinations of 6-benzylaminopurine and 1-naphthaleneacetic acid, callus was initiated, but the growth rate and shoot differentiation differ during 5 subcultures. In general, the highest shoot induction (97%) with maximum increase in fresh and dry weight of culture was obtained on medium supplemented with 0.5 mg L 1 6-benzylaminopurine + 0.2 mg L 1 1-naphthaleneacetic acid after 3 subcultures. On the other hand, benzylaminopurine at 5.0 or 2.0 mg L 1 stimulated growth of callus with very little shoots and accompanied by an increase in fresh and dry weight till 4th and 5th subcultures, respectively. Moreover, when cytokinin (BAP) was added at 1 mg L 1 and auxin (NAA) at 0.2 or 0.1 mg L 1, an increase in shoot production (80%) was obtained after 4 subcultures. Whole plantlets of P. nervosum were recovered following rooting of shoots in MS medium without hormones and successfully re-established in soil under controlled conditions. The relative amounts of the major component of the essential oil of the regenerated shoots, namely menthone, myrtenol, citral, neryl acetate, -muurolene, allo-aromadenderene, -farnesene, -muurolene, -cadiene were different from those present in the partially differentiated callus and parent plant however, citral was the main oil components present in all cultures.
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How to cite this article
Fatma Abd EL-Lateef Gharib, 2005. Changes in Regeneration and Oil Accumulation of Pelargonium nervosum
under Various Culture Conditions. Journal of Biological Sciences, 5: 670-677.
DOI: 10.3923/jbs.2005.670.677
URL: https://scialert.net/abstract/?doi=jbs.2005.670.677
DOI: 10.3923/jbs.2005.670.677
URL: https://scialert.net/abstract/?doi=jbs.2005.670.677
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