Dongli Zhao
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,
Morgan-Tan International Center for Life Sciences, Fudan University,
Shanghai 200433, Peoples Republic of China
Qian Wang
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,
Morgan-Tan International Center for Life Sciences, Fudan University,
Shanghai 200433, Peoples Republic of China
Guoyin Kai
Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,
School of Agriculture and Biology, Shanghai Jiao Tong University,
Shanghai 200030, Peoples Republic of China
Xinglong Wang
Not Available
Juan Lin
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,
Morgan-Tan International Center for Life Sciences, Fudan University,
Shanghai 200433, Peoples Republic of China
Yan Pi
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,
Morgan-Tan International Center for Life Sciences, Fudan University,
Shanghai 200433, Peoples Republic of China
Zhiqi Miao
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,
Morgan-Tan International Center for Life Sciences, Fudan University,
Shanghai 200433, Peoples Republic of China
Xiaofen Sun
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,
Morgan-Tan International Center for Life Sciences, Fudan University,
Shanghai 200433, Peoples Republic of China
Kexuan Tang
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,
Morgan-Tan International Center for Life Sciences, Fudan University,
Shanghai 200433, Peoples Republic of China
ABSTRACT
By RACE-PCR cloning, a cDNA encoding taxane 2α-O-benzoyltransferase (designated as TmTBT) was isolated from Taxus media, which catalyzes the conversion of 2-debenzoyl-7,13-diacetylbaccatin III, a semisynthetic substrate, to 7,13-diacetylbaccatin III. The full-length cDNA of TmTBT was 1478 bp and contained a 1320 bp Open Reading Frame (ORF) encoding a protein of 440 amino acid residues with molecular weight of 50,090 Da and an isoelectric point (pI) of 6.25, similar to taxane 2α-O-benzoyltransferase from Taxus cuspidata. Sequence comparison analysis revealed that TmTBT had high similarity with other members of plant transferase family. Phylogenetic tree analysis showed that TmTBT had close relationship with taxane 2α-O-benzoyltransferase from T. cuspidata. Tissue expression pattern analysis revealed that TmTBT expressed only in leaves and no expression could be detected in fruits and stems, indicating that TmTBT was a tissue-specific gene.
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How to cite this article
Dongli Zhao, Qian Wang, Guoyin Kai, Xinglong Wang, Juan Lin, Yan Pi, Zhiqi Miao, Xiaofen Sun and Kexuan Tang, 2006. Molecular Cloning and Characterization of cDNA Encoding Taxane 2α-O-benzoyltransferase, Catalyzing Taxol Biosynthesis from Taxus media. Journal of Biological Sciences, 6: 651-658.
DOI: 10.3923/jbs.2006.651.658
URL: https://scialert.net/abstract/?doi=jbs.2006.651.658
DOI: 10.3923/jbs.2006.651.658
URL: https://scialert.net/abstract/?doi=jbs.2006.651.658
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