Halima Hassan Salem
Department of Cell Biology and Medical Genetics, Shantou University, Medical College,
22 XinLing Road, Shantou, Guangdong 515031, Peoples Republic of China
Tian-Hua Huang
Department of Cell Biology and Medical Genetics, Shantou University, Medical College,
22 XinLing Road, Shantou, Guangdong 515031, Peoples Republic of China
Bahy Ahmed Ali
Department of Nucleic Acid Research, Genetic Engineering and Biotechnology Research Institute,
Mubarak City for Scientific Research and Technology Applications, New Borg El-Arab City 21934, Alexandria, Egypt
Qing-Dong Xie
Department of Cell Biology and Medical Genetics, Shantou University, Medical College,
22 XinLing Road, Shantou, Guangdong 515031, Peoples Republic of China
ABSTRACT
The aim of this study was to evaluate the genetic similarity and phylogenetic relationship among four Bacillus thuringiensis subspecies (new isolate and three reference strains). Random amplified polymorphic DNA (RAPD) technique was used in this study. Based on RAPD pattern obtained with total of seventeen random primers, the similarity index was calculated for each primer separately and the average for all primers was carried out with each comparison. Moreover, the scored band data was subjected to cluster analysis using Statistica/W5.0 Software package. Based on RAPD data obtained with all primers, the average of genetic similarities between the new isolate (66) and ACCC 10061, tt4 and i977 was 42.47; 51.10; and 45.90, respectively. The four strains are grouped into two major clusters A and B. Cluster A consisted of B. thuringiensis Berliner (ACCC 10061) strain and cluster B consisted of the three B. thuringiensis (66, tt4 and i977) strains. RAPD-PCR could be used extensively to study phylogenetic relationship among B. thuringiensis strains from different subspecies as well as for identification new isolate bacterial strains.
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How to cite this article
Halima Hassan Salem, Tian-Hua Huang, Bahy Ahmed Ali and Qing-Dong Xie, 2006. Genetic Similarity Among Four Bacillus thuringiensis subspecies Based on Random Amplified Polymorphic DNA (RAPD). Journal of Biological Sciences, 6: 781-786.
DOI: 10.3923/jbs.2006.781.786
URL: https://scialert.net/abstract/?doi=jbs.2006.781.786
DOI: 10.3923/jbs.2006.781.786
URL: https://scialert.net/abstract/?doi=jbs.2006.781.786
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