N. Iqbal
Nuclear Institute for Agriculture and Biology (NIAB),
P. 0. Box 128, Jhang Road Faisalabad, Pakistan
S. M. Reader
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
P.D. S. Caligari
Department of Agricultural Botany, School of Plant Sciences, University of Reading, Whiteknights,
Reading RG6 6AS, UK
T. E. Miller
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
ABSTRACT
Previously reported genomic in situ hybridization protocols failed to differentiate Aegilops uniaristata chromosomes in a polyploid wheat background. Different protocols and hybridization temperatures were tested to optimize the differentiation of N genome A. uniaristata chromosomes from those of the A, B and D genomes of wheat. A combination of pre-hybridization of cytological preparations, pre-annealing of the genomic probe with blocking DNA and hybridization at elevated temperatures proved successful. A genomic in situ hybridization protocol for the differentiation of chromosomes from very closely related genomes in a polyploid background is reported.
PDF References Citation
How to cite this article
N. Iqbal, S. M. Reader, P.D. S. Caligari and T. E. Miller, 2002. An Improved Genomic In situ Hybridization (GISH) Method to Differentiate Chromosomes from Closely Related Genomes. Pakistan Journal of Biological Sciences, 5: 869-870.
DOI: 10.3923/pjbs.2002.869.870
URL: https://scialert.net/abstract/?doi=pjbs.2002.869.870
DOI: 10.3923/pjbs.2002.869.870
URL: https://scialert.net/abstract/?doi=pjbs.2002.869.870
REFERENCES
- Schwarzacher, T., A.R. Leitch, M.D. Bennett and J.S. Heslop-Harrison, 1989. In situ localization of parental genomes in wide hybrid. Ann. Bot., 64: 315-324.
Direct Link