R. Mahmud
Department of Dairy and Poultry Science, Chittagong Government Veterinary College,
Pahartali, Chittagong-4202, Bangladesh
M. A. Matin
Department of Dairy and Poultry Science, Chittagong Government Veterinary College,
Pahartali, Chittagong-4202, Bangladesh
H. Otani
Laboratory of Food Bioscience, Graduate School of Agriculture, Shinshu University,
8304 Minamiminowa-mura, Nagana-ken 399-4598, Japan
ABSTRACT
Bovine milk β-lactoglobulin was found to induce proliferation of mouse resting spleen cells. Hence, the mitogenic activity was investigated in detail. The cell growth activity was measured by a colorimetric assay using 3-(4, 5-dimethylthiazol-2-yl)-2-5 diphenyl tetrazolium bromide (MTT). A reduced S-carboxymethylated β-lactoglobulin retained mitogenic activity towards mouse spleen cells and the mitogenic activity increased noticeably after digestion with gastrointestinal proteinases, in particular pancreatin. The pancreat n digest also induced proliferation of U266 cells, a human B cell line while it little influenced the growth of a human B cell line, Ball and human T cell lines Jurkat or Molt-3. These results suggest that bovine β-lactoglobulin may involve a mitogenic sequence(s) against some lymphocytes by the action of intestinal proteinases.
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How to cite this article
R. Mahmud, M. A. Matin and H. Otani, 2004. Mitogenic Effect of Bovine β-lactoglobulin and its Proteolytic Digests
on Mouse Spleen Resting Cells. Pakistan Journal of Biological Sciences, 7: 2045-2050.
DOI: 10.3923/pjbs.2004.2045.2050
URL: https://scialert.net/abstract/?doi=pjbs.2004.2045.2050
DOI: 10.3923/pjbs.2004.2045.2050
URL: https://scialert.net/abstract/?doi=pjbs.2004.2045.2050
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