Kishiko Okada
The United Graduate School of Agricultural Sciences, Ehime University,
3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan
Sanro Tachibana
Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan
Masaya Shiraishi
Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan
ABSTRACT
Starch grains in callus cells of Taxus cuspidata var. nana were clearly detectable when the vesicles containing Taxol-like substances in the cells were qualitatively stained with iodine-potassium iodide reagent solution. Moreover, from observations made under a transmission electron microscope, the vesicles containing starch grains were demonstrated to be plastids wrapped with a double membrane. The plastids were divided roughly into proplastids, leucoplasts and amyloplasts. The proplastid had mainly formed around the nucleus in the juvenile callus cells right after the cell division of 1.5 to 2.5-month-old callus cells. Then, the leucoplast that developed from the proplast moved from the nucleus to the vicinity of the vacuole. Furthermore, the leucoplast in 2.5-month-old mature callus cells was scattered around the large-hypertrophied vacuole. The leucoplast containing 1 to 2 starch grains was observed in the plastid of 1.5-month-old juvenile callus cells. Moreover, numerous amyloplasts containing 3 to 5 starch grains were observed in the 2.5 to 3-month-old mature and aged callus cells. The amyloplast began to invaginate into the tonoplast in the aged and browning callus cells and finally it floated in the vacuole.
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How to cite this article
Kishiko Okada, Sanro Tachibana and Masaya Shiraishi, 2004. Electron Microscopic Observation of Plastid Containing Taxol-like Substances
in Callus Cells of Taxus cuspidata var. Nana. Pakistan Journal of Biological Sciences, 7: 2139-2148.
DOI: 10.3923/pjbs.2004.2139.2148
URL: https://scialert.net/abstract/?doi=pjbs.2004.2139.2148
DOI: 10.3923/pjbs.2004.2139.2148
URL: https://scialert.net/abstract/?doi=pjbs.2004.2139.2148
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