Anna Ling Pick Kiong
Department of Bioscience, Faculty of Engineering and Science,
Universiti Tunku Abdul Rahman, 53300 Setapak, Kuala Lumpur, Malaysia
Maziah Mahmood
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science,
Nor’aini Mohd Fadzillah
Department of Biology, Faculty of Science,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia
Siti Khalijah Daud
Department of Biology, Faculty of Science,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia
ABSTRACT
Production of the four targeted triterpenes, asiatic acid, madecassic acid, asiaticoside and madecassoside in leaf derived callus and cell suspension cultures of Centella asiatica was compared with its production in whole plant from field and in vitro shoot cultures. In callus a cultures, production was found the highest during the third week of culture and the contents declined gradually. The glycosides, madecassoside and asiaticoside contents were found higher than the asiatic acid and madecassic acid in callus cultures. Four triterpenes precursors, squalene, Farnesyl Pyrophosphate (FPP), Isopentenyl Pyrophosphate (IPP) and leucine, which are involved directly or indirectly in the triterpene biosynthetic pathway, were used to increase the triterpenes production. Squalene was found the best precursor in promoting triterpenes production in callus cultures. Squalene treated callus also produced the highest biomass production compared to the other precursors tested.
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How to cite this article
Anna Ling Pick Kiong, Maziah Mahmood, Nor’aini Mohd Fadzillah and Siti Khalijah Daud, 2005. Effects of Precursor Supplementation on the Production of Triterpenes by Centella
asiatica Callus Cultures. Pakistan Journal of Biological Sciences, 8: 1160-1169.
DOI: 10.3923/pjbs.2005.1160.1169
URL: https://scialert.net/abstract/?doi=pjbs.2005.1160.1169
DOI: 10.3923/pjbs.2005.1160.1169
URL: https://scialert.net/abstract/?doi=pjbs.2005.1160.1169
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