A. Mesdaghinia
Center for Environmental Research,
S. Rezaie
Department of Parasitology and Mycology, School of Public Health,
Tehran University of Medical Science, Tehran, Iran
M. Shariat
Department of Environmental Health Engineering,
S. Nasseri
Department of Environmental Health Engineering,
A. H. Mahvi
Department of Environmental Health Engineering,
G. A. Omrani
Department of Environmental Health Engineering,
S. Nazmara
Department of Environmental Health Engineering,
A. Ghasri
Department of Environmental Health Engineering,
M. Alimohammadi
Department of Environmental Health Engineering,
ABSTRACT
This research was carried out to isolate and identify degrading microorganisms of MTBE in the soil samples taken from the adjacent ground of lead-free gasoline storage tanks as well as from drainage water of MTBE and gasoline storage tanks. Water and soil samples were prepared and microorganisms were inoculated and then harvested from mineral salt media. After several passages within 200 days, microorganisms capable of using MTBE as carbon and energy source were isolated. Isolated microorganisms inoculated in common culture media including R2A agar and BHI were identified as Pseudomonas putida, Comamonas, Alcaligenes, Bacillus and Micrococcus by specific kit of epi.
PDF References Citation
How to cite this article
A. Mesdaghinia, S. Rezaie, M. Shariat, S. Nasseri, A. H. Mahvi, G. A. Omrani, S. Nazmara, A. Ghasri and M. Alimohammadi, 2005. Isolation and Detection of MTBE Degrading Bacteria. Pakistan Journal of Biological Sciences, 8: 974-977.
DOI: 10.3923/pjbs.2005.974.977
URL: https://scialert.net/abstract/?doi=pjbs.2005.974.977
DOI: 10.3923/pjbs.2005.974.977
URL: https://scialert.net/abstract/?doi=pjbs.2005.974.977
REFERENCES
- Krassimira, H., C.M. Lutenegger and K.M. Scow, 2001. Detection and quantification of methyl tert-butyl ether-degrading strain PM1 by real-time TaqMan PCR. Applied Environ. Microbiol., 67: 5154-5160.
CrossRefDirect Link - Hatzinger, P.B., K. McClay, S. Vainberg, M. Tugusheva, C.W. Condee and R.J. Steffan, 2001. Biodegradation of methyl tert-butyl ether by a pure bacterial culture. Applied Environ. Microbiol., 67: 5601-5607.
CrossRefPubMedDirect Link - Christy, A.S. and M.R. Hyman, 2004. Oxidation of methyl tert-butyl ether by alkane hydroxylase in dicyclopropylketone-induced and N-octane-grown Pseudomonas putida Gpo1. Applied Environ. Microbiol., 70: 4544-4550.
Direct Link - Christy, A.S., K.T. Oreilly and M.R. Hyman, 2003. Cometabolism of methyl tertiary butyl ether and gaseous N-alkanes by Pseudomonas mendocina Kr-1 grown on C5 to C8 N-alkanes. Applied Environ. Microbiol., 50: 7385-7394.
CrossRefDirect Link - Catherine, Y.L., G.E. Speitel and J.G. Georgiou, 2001. Kinetics of methyl tert-butyl ether cometabolism at low concentrations by pure cultures of butane-degrading bacteria. Applied Environ. Microbiol., 67: 2197-2201.
CrossRefDirect Link - Fayolle, A.F., L. Garnier, D. Godefroy, H. Mathis, P. Piveteau and F. Monot, 2003. Limitations in MTBE biodegradation. Oil Gas Sci. Technol., 58: 497-504.
CrossRefDirect Link - Beguin, P., S. Chauvaux, I. Miras, A. Francois, F. Fayolle and F. Monot, 2003. Genes involved in the degradation of ether fuels by bacteria of the Mycobacterium rhodococcus group. Oil Gas Sci. Technol., 58: 489-495.
CrossRefDirect Link - Linda, K.H., S.S. Curry, L.M. Ciuffetti and M.R. Hyman, 1997. Metabolism of diethyl ether and cometabolism of methyl tert-butyl ether by a filamentous fungus, a Graphium sp. Applied Environ. Microbiol., 63: 3059-3067.
Direct Link