Uzoamaka Ogechi George-Okafor
Department of Applied Microbiology and Brewing, Enugu State University of Science and Technology, Agbani-Enugu, Nigeria
Enuma Ekpereka Anosike
Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
ABSTRACT
Lactic Acid Bacteria (LAB) as probiotic organisms can be sourced from fermented diary and vegetable products. The problem in Nigeria is their affordability to the greater populace, as the common sources are relatively expensive. The study was aimed at sourcing for a low cost meal rich in probiotic LAB by utilizing raw lime juice for the fermentation of corn. Washed yellow corn variety (6%) was fermented using prepared lime solution (pH 5.1-5.5). The lime-fermented corn was wet-milled, sieved and the resulting substrate was enumerated for LAB and coliform on DeMan Rogosa Sharpe agar and MacConkey agar plates respectively. The predominant LAB were characterized as Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus acidophilus. At their predetermined optima growth periods (18 h, 22 h and 24 h), they were evaluated for antimicrobial potentials against Salmonella typhi, Shigella dysenteriae and Candida albicans. Their resistance to acid, bile and antibiotics was determined. The LAB (>3.80 x 108±012 cfu/ml) recovered from lime-treated samples were significantly (p<0.05) higher than that from untreated samples (<5.70 x 106±003 cfu/ml). The pH (<5.3) and coliform counts (<0.50 x 101±013) were also reduced in the treated sample. Although their antibiotic resistance was very low, the test LAB especially L. acidophilus demonstrated efficient probiotic properties by inhibiting the test pathogens, maintaining >78% acid stability at pH 3 and withstanding up to 20% bile concentration at 51.4% survival rate. These results reveal that low cost lime-fermented corn is a rich source for probiotic LAB and thus can be recommended as a food supplement.
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How to cite this article
Uzoamaka Ogechi George-Okafor and Enuma Ekpereka Anosike, 2010. The Potentials of Lime (Citrus aurantifolia) for Improving Traditional Corn Fermentation for Probiotic Lactic Acid Bacterial Proliferation. Pakistan Journal of Nutrition, 9: 1207-1213.
DOI: 10.3923/pjn.2010.1207.1213
URL: https://scialert.net/abstract/?doi=pjn.2010.1207.1213
DOI: 10.3923/pjn.2010.1207.1213
URL: https://scialert.net/abstract/?doi=pjn.2010.1207.1213
REFERENCES
- Achi, O.K., 2005. The potentials for upgrading traditional fermented foods through biotechnology. Afr. J. Biotechnol., 4: 375-380.
Direct Link - Adeyemi, I.A. and O. Beckkley, 1986. Effect of period of maize fermentation and souring on chemical properties and amylograph pasting viscosity of Ogi. J. Cereal Sci., 4: 353-360.
CrossRefDirect Link - Ahn, Y.T., G.B. Kim, K.S. Lim, Y.J. Baek and H.U. Kim, 2003. Deconjugation of bile salts by Lactobacillus acidophilus isolate. Int. Dairy J., 13: 303-311.
CrossRef - Amusa, N.A., O.A. Ashaye and M.O. Oladapo, 2005. Microbiological quality of Ogi and Soy-Ogi (a Nigerian fermented cereal porridge) widely consumed and notable weaning food in Southern Nigeria. J. Food Agric. Environ., 3: 81-83.
Direct Link - Anukam, K.C., E.O. Osazuwa, B.E. Osadolor, A.W. Bruce and G. Reid, 2008. Yogurt containing probiotic Lactobacillus rhamnosus GR-1 and L. reuteri RC-14 helps resolve moderate diarrhea and increases CD4 count in HIV/AIDS patients. J. Clin. Gastroenterol., 42: 239-243.
Direct Link - Ashar, M.A. and J.B. Prajapathi, 2000. Verification of hyocholesterolemic effect of fermented milk on human subjects with different cholesterol levels. Folia Microbiol., 45: 263-268.
CrossRefPubMedDirect Link - Brady, L.J., D.D. Gallaher and F.F. Busta, 2000. The role of probiotic cultures in the prevention of colon cancer. J. Nutr., 130: 410S-414S.
PubMedDirect Link - Brizuela, M.A., P. Serrano and Y. Perez, 2001. Studies on probiotics properties of two Lactobacillus strains. Brazil. Arch. Biol. Technol., 44: 1-8.
CrossRef - Coconnier, M.H., V. Lievin, M. Lorrot and A.L. Servin, 2000. Antagonistic activity of Lactobacillus acidophilus LB against intracellular Salmonella enterica serovar Typhimurium infecting human enterocyte-like Caco-2/Tc-7 cells. Applied Environ. Microbiol., 66: 1152-1157.
PubMedDirect Link - Denou, E., R.D. Pridmore, B. Berger, J.M. Panoff, F. Arigoni and H. Brussow, 2008. Identification of genes associated with the long-gut-persistence phenotype of the probiotic Lactobacillus johnsonii strain NCC533, using a combination of genomics and transcriptome analysis. J. Bacteriol., 180: 3161-3163.
CrossRefPubMed - Dunne, C., L. O'Mahony, L. Murphy, G. Thornton and D. Morrissey et al., 2001. In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin. Nutr., 73: 386s-392s.
PubMedDirect Link - Gueimonde, M., L. Jalonen, F. He, M. Hiramatsu and S. Salminen, 2006. Adhesion and competitive inhibition and displacement of human enteropathogens by selected lactobacilli. Food Res. Int., 39: 467-471.
CrossRef - Herrero, M., B. Mayo, B. Gonzalez and J.E. Suarez, 1996. Evaluation of technologically important traits in lactic acid bacteria isolated from spontaneous fermentation. J. Applied Bacteriol., 82: 565-570.
Direct Link - Holt, J.G., N.R. Kreig, P.H.A. Sneath, J.T. Staley and S.T. Williams, 1994. Bergey's Manual of Determinative Bacteriology. 9th Edn., Lippincott Williams and Wilkins, Baltimore, USA., ISBN-13: 9780683006032, Pages: 787.
Direct Link - Lee, Y.K. and S. Salminen, 1995. The coming of age of probiotics. Trends Food Sci. Technol., 6: 241-245.
CrossRef - Lorca, G.L. and G.F. de Valdez, 2001. Acid tolerance mediated by membrane ATPases in Lactobacillus acidophilus. Biotechnol. Letters, 23: 777-780.
CrossRef - Mattila-Sandholm, T., J. Matto and M. Saarela, 1999. Lactic acid bacteria with health claims-interactions and interference with gastrointestinal flora. Int. Dairy J., 91: 25-35.
CrossRef - McAuliffe, O., R.J. Cano and T.R. Klaenhammer, 2005. Genetic analysis of two bile salt hydrolase activities in Lactobacillus acidophilus NCFM. Applied Environ. Microbiol., 71: 4925-4929.
CrossRefPubMedDirect Link - McCarthy, J., L. O`Mahony, L. O`Callaghan, B. Shell and E.E. Vaughan et al., 2003. Double blind, placebo controlled trial of two probiotic strains in interleukin 10 knockout mice and mechanism link with cytokine balance. Gut, 52: 975-980.
PubMedDirect Link - Ouwehand, A.C., S. Salminen and E. Isolauri, 2002. Probiotics: An overview of beneficial effects. Antonie Van Leeuwenhoek, 82: 279-289.
PubMedDirect Link - Saxelin, M., B. Grenov, U. Svensson, R. Fonden, R. Reniero and T. Mattila-Sandholm, 1999. The technology of probiotics. Trends Food Sci. Technol., 10: 387-392.
CrossRefDirect Link - Sefa-Dedeh, S., B. Cornelius, W. Amoa-Awua, E. Sakyi-Dawson and E.O. Ajoakwa, 2004. The microflora of fermented nixtamalized corn. Int. J. Food Microbiol., 96: 97-102.
Direct Link - Simakachorn, N., V. Pichaipat, P. Rithiporn, C. Kongkaeuw, P. Tongpradit and W. Varavithya, 2000. Clinical evaluation of the addition of lyophilized, heat-killed Lactobacillus acidophilus LB to oral rehydration therapy in the treatment of acute diarrhea in children. J. Ped. Gastroentrol. Nutr., 30: 68-72.
Direct Link - Soomro, A.H., T. Masud and K. Anwaar, 2002. Role of Lactic Acid Bacteria (LAB) in food preservation and human health-A review. Pak. J. Nutr., 1: 20-24.
CrossRefDirect Link - Wang, K.Y., S.N. Li, C.S. Liu, D.S. Perng and Y.C. Su et al., 2004. Effects of ingesting Lactobacillus and Bifidobacterium-containing yogurt in subjects with colonized Helicobacter pylori. Am. J. Clin. Nutr., 80: 737-741.
PubMedDirect Link - Weizman, Z., A. Ghaleb and A. Alsheikh, 2005. Effect of a probiotic infant formula on infections in child care centers: Comparison of two probiotic agents. Pediatrics, 115: 5-9.
Direct Link - Wollowski, I., G. Rechkemmer and B.L. Pool-Zobel, 2001. Protective role of probiotics and prebiotics in colon cancer. Am. J. Clin. Nutr., 73: 451s-455s.
PubMedDirect Link - Charteris, W.P., P.M. Kelly, L. Morelli and J.K. Collins, 1998. Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract. J. Appl. Microbiol., 84: 759-768.
CrossRefPubMedDirect Link