Katrin Roosita
Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Bogor, Indonesia
Rimbawan Sukandar Martopuro
Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Bogor, Indonesia
Ita Djuwita
Laboratory of Embryology, Veterinary Faculty, Bogor Agricultural University (IPB), Bogor, Indonesia
M. Rizal Damanik
Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Bogor, Indonesia
Clara M. Kusharto
Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Bogor, Indonesia
Fumito Tani
Laboratory of Food and Environmental Science, Division of Food Science and Biotechnology, Faculty of Agriculture, Kyoto University, Japan
ABSTRACT
β-carotene is the most widely studied carotenoid that has provitamin A properties beside its great promise for cancer chemoprotective and human physiological functions. β-carotene can be cleavaged to retinal by some enzymes in wide variety of cells especially in intestinal cells. The retinal is further metabolized to retinoic acid by Aldehyde Dehydrogenase (ALDH) family of enzymes. In this study we use CMT-93 cell line as a cell model of intestinal epithelial cell to study the effect of β-carotene on cells morphology, proliferation and Aldehyde Dehydrogenase (ALDH) gene expression. These cells were grown in DMEM supplemented with 10% heat-inactivated Fecal Bovine Serum (FBS), 4 mM L-glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin. Tetrahydrofuran (THF) (1.25%) was used to solve β-carotene (Sigma). Final concentration of β-carotene in medium for proliferation were 0.5, 1.0, 1.5 and 5.0 μM. The results showed that in higher concentration (1.5 μM), β-carotene can alter the morphologycal structure of monolayer epithelial cells of CMT-93. Meanwhile, β-carotene in supra physiological concentration (5.0 μM) significantly (p<0.05) decreased proliferation, exhibited ALDH1A2 gene expression and changed morphological structure.
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How to cite this article
Katrin Roosita, Rimbawan Sukandar Martopuro, Ita Djuwita, M. Rizal Damanik, Clara M. Kusharto and Fumito Tani, 2013. Biological Response of Epithelial Cells line (CMT-93) Induced by β-Carotene. Pakistan Journal of Nutrition, 12: 615-619.
DOI: 10.3923/pjn.2013.615.619
URL: https://scialert.net/abstract/?doi=pjn.2013.615.619
DOI: 10.3923/pjn.2013.615.619
URL: https://scialert.net/abstract/?doi=pjn.2013.615.619
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